
J. Manage. Hum. Resour. (July - December 2026) 4(2):
Introduction
The term "Biosafety" encompasses regulations and precautions
designed to protect human health against biological, physical,
chemical, and other occupational risks while supporting safe care
environments. In healthcare, infection prevention and control depends
on the consistent application of standard precautions, including hand
hygiene and the appropriate use of personal protective equipment
(PPE) (World Health Organization [WHO], 2022). Compliance with
protocols, techniques, and appropriate use of resources is therefore a
fundamental component of biosafety.
Personal protective equipment (PPE) comprises barriers used alone
or in combination to protect the skin, respiratory and other mucous
membranes, eyes, and clothing from exposure to potentially
infectious material. PPE selection depends on the expected interaction
with patients and the mechanism of transmission, and its effectiveness
relies on correct selection, donning, use, and removal (Moris Vidal et
al., 2021; Verbeek et al., 2020).
Healthcare-associated infections remain a major patient- and
worker-safety challenge. The World Health Organization reports that
infection prevention and control programs, including appropriate
hand hygiene and related precautions, can prevent a substantial
proportion of healthcare-associated infections (WHO, 2022). These
findings reinforce the importance of adherence to biosafety measures
among healthcare professionals.
Healthcare workers are exposed to occupational infections through
contact with patients, contaminated materials, body fluids, and
respiratory hazards. Standard precautions—particularly hand hygiene,
respiratory hygiene, safe handling of healthcare waste, and
appropriate PPE—are central to reducing these risks (WHO, 2022).
Evidence from the COVID-19 period also showed that deficiencies in
infection-prevention measures and PPE availability can increase
occupational risk among healthcare personnel (Palacio Lapuente et
al., 2021).
The evaluation of personal protective equipment (PPE) use among
healthcare professionals reveals important challenges in adherence
and perception. In Ecuador, Cordero Ramirez et al. (2024) found that
48.3% of nurses had a poor perception of PPE use, while workplace
motivation showed a positive relationship with professional
performance. Evidence also indicates that appropriate masks and
other PPE contribute to reducing exposure to respiratory and highly
infectious diseases (Chou et al., 2020; Verbeek et al., 2020).
However, adherence depends not only on equipment availability but
also on knowledge, training, organizational support, and compliance
with standard precautions (De Sousa et al., 2019; Palacio Lapuente et
al., 2021).
This study is highly relevant in the healthcare setting, as all
healthcare professionals must be familiar with the protocols for the
correct use of biosafety measures. These protocols play a vital role in
preventing hospital-acquired infections and reducing occupational
risks. This research will yield valuable and up-to-date data that will
allow for a better understanding of compliance with these protocols
by all healthcare professionals at this prestigious institution.
Methodology
This study is classified as descriptive, meaning its main objective
is to provide a detailed overview of the characteristics and conditions
of the selected population. A quantitative method is employed,
allowing for the collection and analysis of numerical data, facilitating
the identification of patterns and trends. Furthermore, the study
design is prospective, meaning that data will be collected over time,
specifically in a cross-sectional period. This methodology is suitable
for capturing a current and specific picture of the use of personal
protective equipment (PPE) in a hospital setting. The target
population consists of 36 healthcare workers, representing a
significant sample of the staff working at a hospital in Manabí. From
this population, 32 workers were selected from various areas where
PPE is used. This selection ensures adequate representation that
reflects the diverse realities and needs of healthcare professionals
within the hospital context.
The inclusion criteria are fundamental to the rigor of the study,
allowing only those professionals who voluntarily agree to participate
to do so. This decision ensures that the data collected is relevant and
that the participants are willing to collaborate in the research. In
contrast, the exclusion criteria were based on excluding those workers
who did not wish to participate, which reinforces the ethical standards
and respect for the individuals involved.
Procedure for collecting information
The data collection technique used was a validated survey,
employing indirect observation to gather relevant information on the
use of personal protective equipment. The data collection instrument
was a checklist, which facilitated direct observation during May,
June, and July of 2025, obtaining precise and specific data within a
defined period, thus allowing for a better interpretation of the results.
Data processing and analysis were carried out using Microsoft Excel,
and the results were subsequently represented in graphs, facilitating
the understanding of the findings. This quantitative approach ensures
that the results are objective and easily interpretable.
Regarding ethical procedures, informed consent was obtained from
all participants, ensuring they understood the nature of the study and
their right to participate voluntarily. Furthermore, permission was
requested from teaching managers and department heads to conduct
direct observation. The information collected was used exclusively
for academic purposes, guaranteeing the absence of conflicts of
interest and respecting the privacy and integrity of the participants.
Results and Discussion
Figure 1. Work area
The geographical distribution of staff indicates that the majority of
respondents work in high-demand, high-complexity units. Internal
Medicine accounts for 28.1%, Emergency Medicine for 25% (53.1%
combined), reflecting the reality of frontline staff who manage both
complex cases requiring prolonged hospitalization and the high
volume of emergency and critical patients. In addition to primary care
units, the operating room contributes 15.6%, while Infectious
Diseases and the Intensive Care Unit (ICU) account for 12.5%,
establishing a profile of staff who are highly exposed and directly
responsible for implementing infection control measures in high-risk
environments.
Figure 2. Professional activity of the study population
J. Manage. Hum. Resour. (July - December 2026) 4(2): 1-5 2
Introduction
The term "Biosafety" encompasses regulations and precautions
designed to protect human health against biological, physical,
chemical, and other occupational risks while supporting safe care
environments. In healthcare, infection prevention and control depends
on the consistent application of standard precautions, including hand
hygiene and the appropriate use of personal protective equipment
(PPE) (World Health Organization [WHO], 2022). Compliance with
protocols, techniques, and appropriate use of resources is therefore a
fundamental component of biosafety.
Personal protective equipment (PPE) comprises barriers used alone
or in combination to protect the skin, respiratory and other mucous
membranes, eyes, and clothing from exposure to potentially
infectious material. PPE selection depends on the expected interaction
with patients and the mechanism of transmission, and its effectiveness
relies on correct selection, donning, use, and removal (Moris Vidal et
al., 2021; Verbeek et al., 2020).
Healthcare-associated infections remain a major patient- and
worker-safety challenge. The World Health Organization reports that
infection prevention and control programs, including appropriate
hand hygiene and related precautions, can prevent a substantial
proportion of healthcare-associated infections (WHO, 2022). These
findings reinforce the importance of adherence to biosafety measures
among healthcare professionals.
Healthcare workers are exposed to occupational infections through
contact with patients, contaminated materials, body fluids, and
respiratory hazards. Standard precautions—particularly hand hygiene,
respiratory hygiene, safe handling of healthcare waste, and
appropriate PPE—are central to reducing these risks (WHO, 2022).
Evidence from the COVID-19 period also showed that deficiencies in
infection-prevention measures and PPE availability can increase
occupational risk among healthcare personnel (Palacio Lapuente et
al., 2021).
The evaluation of personal protective equipment (PPE) use among
healthcare professionals reveals important challenges in adherence
and perception. In Ecuador, Cordero Ramirez et al. (2024) found that
48.3% of nurses had a poor perception of PPE use, while workplace
motivation showed a positive relationship with professional
performance. Evidence also indicates that appropriate masks and
other PPE contribute to reducing exposure to respiratory and highly
infectious diseases (Chou et al., 2020; Verbeek et al., 2020).
However, adherence depends not only on equipment availability but
also on knowledge, training, organizational support, and compliance
with standard precautions (De Sousa et al., 2019; Palacio Lapuente et
al., 2021).
This study is highly relevant in the healthcare setting, as all
healthcare professionals must be familiar with the protocols for the
correct use of biosafety measures. These protocols play a vital role in
preventing hospital-acquired infections and reducing occupational
risks. This research will yield valuable and up-to-date data that will
allow for a better understanding of compliance with these protocols
by all healthcare professionals at this prestigious institution.
Methodology
This study is classified as descriptive, meaning its main objective
is to provide a detailed overview of the characteristics and conditions
of the selected population. A quantitative method is employed,
allowing for the collection and analysis of numerical data, facilitating
the identification of patterns and trends. Furthermore, the study
design is prospective, meaning that data will be collected over time,
specifically in a cross-sectional period. This methodology is suitable
for capturing a current and specific picture of the us personal
protective equipment (PPE) in a hospital setting. The target
population consists of 36 healthcare workers, representing a
significant sample of the staff working at a hospital in Manabí. From
this population, 32 workers were selected from various areas where
PPE is used. This selection ensures adequate representation that
reflects the diverse realities and needs of healthcare professionals
within the hospital context.
The inclusion criteria are fundamental to the rigor of the study,
allowing only those professionals who voluntarily agree to participate
to do so. This decision ensures that the data collected is relevant and
that the participants are willing to collaborate in the research. In
contrast, the exclusion criteria were based on excluding those workers
who did not wish to participate, which reinforces the ethical standards
and respect for the individuals involved.
Procedure for collecting information
The data collection technique used was a validated survey,
employing indirect observation to gather relevant information on the
use of personal protective equipment. The data collection instrument
was a checklist, which facilitated direct observation during May,
June, and July of 2025, obtaining precise and specific data within a
defined period, thus allowing for a better interpretation of the results.
Data processing and analysis were carried out using Microsoft Excel,
and the results were subsequently represented in graphs, facilitating
the understanding of the findings. This quantitative approach ensures
that the results are objective and easily interpretable.
Regarding ethical procedures, informed consent was obtained from
all participants, ensuring they understood the nature of the study and
their right to participate voluntarily. Furthermore, permission was
requested from teaching managers and department heads to conduct
direct observation. The information collected was used exclusively
for academic purposes, guaranteeing the absence of conflicts of
interest and respecting the privacy and integrity of the participants.
Results and Discussion
Figure 1. Work area
The geographical distribution of staff indicates that the majority of
respondents work in high-demand, high-complexity units. Internal
Medicine accounts for 28.1%, Emergency Medicine for 25% (53.1%
combined), reflecting the reality of frontline staff who manage both
complex cases requiring prolonged hospitalization and the high
volume of emergency and critical patients. In addition to primary care
units, the operating room contributes 15.6%, while Infectious
Diseases and the Intensive Care Unit (ICU) account for 12.5%,
establishing a profile of staff who are highly exposed and directly
responsible for implementing infection control measures in high-risk
environments.
Figure 2. Professional activity of the study population
3 J. Manage. Hum. Resour. (July - December 2026) 4(2): 1-5
The distribution of professional activity shows that nursing staff
constitute the largest segment of the analyzed population,
representing 37.5%. When combined with nursing interns (18.7%)
and nursing assistants (9.3%), the nursing and related fields group
comprises the absolute majority of the sample (65.5% in total).
Physicians represent the next largest group, with 28.1% of the studied
population, although they are a minority compared to nursing staff.
Finally, 6.2% of the sample is classified as "Other."
Chart 3. Service time
The sample is characterized by a significant degree of seniority and
experience, with the majority of staff having intermediate experience
of 5 to 10 years, comprising 40.6% of the population. This is followed
by the most experienced staff, with more than 10 years of service, at
37.5%. The observed successes or failures in adherence to PPE
practices should be interpreted as reflecting systemic problems or
persistent shortcomings in PPE quality or ongoing training, rather
than simply user inexperience. On the other hand, those with less than
5 years of service are a minority, representing 21.8%. If this younger
group had received more recent training with updated protocols, its
positive impact could be moderated by the influence of the large
number of staff with decades of experience.
Chart 4A. Attitudes of healthcare personnel towards PPE
The most prominent attitude is the strong support for mandatory
N95 masks or equivalent, with 71.8% of respondents believing that
this high-filtration respiratory protection should be mandatory.
Regarding the integration of PPE with other safety protocols, the
majority of respondents agree that PPE should be used in combination
with other precautionary measures, such as hand hygiene, reaching
56.2% agreement. A significant number of respondents disagreed
(12.5% disagreed, 18.7% somewhat agreed or somewhat disagreed,
and 9.3% neither agreed nor disagreed). While staff value hand
hygiene, there is a lack of widespread awareness of the synergistic
nature of infection control measures, where PPE acts as a barrier but
is ineffective without the strict practice of hand hygiene before and
after use.
The appropriate use of personal protective equipment (PPE) is
essential to ensure the safety of healthcare personnel, especially in
high-risk infection settings. According to the findings, there is strong
support for mandatory N95 mask use, with 71.8% of respondents in
favor. This finding is consistent with Chou et al. (2020), who
emphasize the protective role of appropriate masks in healthcare
settings. However, effective protection also depends on correct use,
risk assessment, and adherence to broader infection-prevention
measures, which reinforces the importance of continuous training
(Verbeek et al., 2020).
Chart 4B. Attitudes of healthcare personnel towards PPE
The responses are notably divided, with 40.6% answering 'Yes',
15.6% 'No', 15.6% 'Somewhat agree' or 'Somewhat disagree' and 25%
respectively, and 3.1% 'Neither agree nor disagree'. Regarding
whether the use of PPE will keep healthcare workers safe from
contracting any type of infection, the most common response is 'No',
supported by 34.3%, while only 46.8% answer 'Yes'.
On the other hand, concerns about the effectiveness of reusable
PPE are highlighted in the analysis presented, with 88% of
respondents indicating their use of reusable PPE. Reuse may be
appropriate only when reprocessing or decontamination procedures
are explicitly supported for the specific equipment and are performed
under established protocols; otherwise, protection can be
compromised (WHO, 2020). This suggests that, despite a positive
attitude toward training (82%), practical implementation may be
insufficient, which is consistent with Verbeek et al. (2020), who
emphasize the importance of training in correct PPE use, donning,
and doffing.
Figure 5A. Healthcare personnel practices regarding PPE
First, 81.27% report disinfecting their gloves after each interaction
with a patient. However, a disparity is revealed in the application of
other hygiene measures. For example, the use of goggles and/or face
shields during patient care shows a considerably lower percentage at
65.6%, indicating a lack of awareness about the risk of exposure to
aerosols or bodily fluids, or a misperception of the need for such
measures. The fact that only 56.2% of respondents practice hand
hygiene before and after removing personal protective equipment
(PPE) is equally concerning, as this practice is important for
minimizing the risk of cross-contamination. Furthermore, the use of
shoe covers and biohazard bags appears to be even less common, at
34.3% and 37.5% respectively, raising questions about safety training
and culture in the workplace.
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