Extraction methods of bioactive compounds: a sustainability approach
DOI:
https://doi.org/10.5281/zenodo.14610634Keywords:
extraction methods, green extraction processes, bioactive compounds, environmental sustainability, antioxidants, flavonoidsAbstract
This article provides an overview of various extraction methods used to obtain bioactive compounds from plant materials, highlighting the process conditions, extract properties, and potential applications. Methodologies such as ultrasonic-assisted extraction (UAE), Soxhlet, supercritical fluids extraction (SFE), and green or environmentally friendly methods were studied. Each technique was evaluated in terms of efficiency, cost, environmental impact, and application, considering factors such as the type of compounds extracted (antioxidants, flavonoids, essential oils) and their use in the food, cosmetic, and pharmaceutical industries. The advantages and limitations of each process were discussed, providing a framework for selecting the most suitable method based on specific extraction and sustainability needs.
References
Alcázar-Alay, S., & Gallón-Bedoya, M. (2023). New trends in supercritical fluid technology and pressurized liquids for the extraction and recovery of bioactive compounds from agro-industrial and marine food waste. Molecules, 28(11), 4421. https://doi.org/10.3390/molecules28114421
Aldughaylibi, F.S., Raza, M.A., Naeem, S., Rafi, H., Alam, M.W., Souayeh, B., Farhan, M., Aamir, M., Zaidi, N., & Mir, T.A. (2022). Extraction of bioactive compounds for antioxidant, antimicrobial, and antidiabetic applications. Molecules, 27(18), 5935. https://doi.org/10.3390/molecules27185935
Almohasin, J.A., Balag, J., Miral, V.G., Moreno, R.V., Tongco, L.J., & Lopez, E.C.R. (2023). Green solvents for liquid-liquid extraction: recent advances and future trends. Engineering Proceedings, 56(1), 174. https://doi.org/10.3390/ASEC2023-16278
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., & Lightfoot, D.A. (2017). Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4), 42. https://doi.org/10.3390/plants6040042
Semerdjieva, I.B., Shiwakoti, S., Cantrell, C.L., Zheljazkov, V.D., Astatkie, T., Schlegel, V., & Radoukova, T. (2019). Hydrodistillation extraction kinetics regression models for essential oil yield and composition in Juniperus virginiana, J. excelsa, and J. sabina. Molecules, 24(5), 986. https://doi.org/10.3390/molecules24050986
Borges, A, Homem, V., & Simões, M. (2020). Comparison of techniques and solvents on the antimicrobial and antioxidant potential of extracts from acacia dealbata and olea europaea. Antibiotics (Basel), 9(2):48. https://doi.org/10.3390/antibiotics9020048
Dobrinčić, A., Repajić, M., Garofulić, I. E., Tuđen, L., Dragović-Uzelac, V., & Levaj, B. (2020). Comparison of different extraction methods for the recovery of olive leaves polyphenols. Processes, 8(9), 1008. https://doi.org/10.3390/pr8091008
El Maaiden, E., Bouzroud, S., Nasser, B., Moustaid, K., El Mouttaqi, A., Ibourki, M., Boukcim, H., Hirich, A., Kouisni, L., & El Kharrassi, Y. (2022). A comparative study between conventional and advanced extraction techniques: pharmaceutical and cosmetic properties of plant extracts. Molecules, 27(7), 2074. https://doi.org/10.3390/molecules27072074
Gonelimali, F.D., Lin, J., Miao, W., Xuan, J., Charles, F., Chen, M., & Hatab, S.R. (2018). Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Frontiers Microbiology, 9. https://doi.org/10.3389/fmicb.2018.01639
Fernández, F., Quiñones, L., Ojeda, T.S., Alfaro, D.P., Yalta, R.J., Cabrejos, E.M., & Huamán, A. (2024). Rendimiento de extracción por hidrodestilación del aceite esencial de Piper aduncum L. (matico) mediante un modelo cinético. Revista Científica Dékamu Agropec, 5(1), 33–44. https://doi.org/10.55996/dekamuagropec.v5i1.208
Teixeira, F., Silva, A.M., Sut, S., Dall'Acqua, S., Ramos, O.L., Ribeiro, A.B., Ferraz, R., Delerue-Matos, C., & Rodrigues, F. (2024). Ultrasound-assisted extraction of bioactive compounds from goji berries: Optimization, bioactivity, and intestinal permeability assessment. Food Research International, 188, 114502. https://doi.org/10.1016/j.foodres.2024.114502
Gligor, O., Clichici, S., Moldovan, R., Muntean, D., Vlase, A.M., Nadăș, G.C., Matei, I.A., Filip, G.A., Vlase, L., & Crișan, G. (2023). The effect of extraction methods on phytochemicals and biological activities of green coffee beans extracts. Plants, 12(4), 712. https://doi.org/10.3390/plants12040712
Wong-Paz, J.E., Aguilar-Zárate, P., Veana, F., & Muñiz-Márquez, D.B. (2020). Impacto de las tecnologías de extracción verdes para la obtención de compuestos bioactivos de los residuos de frutos cítricos. TIP. Revista especializada en ciencias químico-biológicas, 23, e20200255. https://doi.org/10.22201/fesz.23958723e.2020.0.255
Junsathian, P., Nakamura, S., Katayama, S., & Rawdkuen, S. (2022). Antioxidant and antimicrobial activities of Thai edible plant extracts prepared using different extraction techniques. Molecules, 27(19), 6489. https://doi.org/10.3390/molecules27196489
Kothari, V., Gupta, A., & Naraniwal, M. (2012). Comparative study of various methods for extraction of antioxidant and antibacterial compounds from plant seeds. Journal of Natural Remedies, 12(2), 162–173. https://doi.org/10.18311/jnr/2012/271
Lavenburg, V.M., Rosentrater, K.A., & Jung, S. (2021). Extraction methods of oils and phytochemicals from seeds and their environmental and economic impacts. Processes, 9(10), 1839. https://doi.org/10.3390/pr9101839
Mahmood, M.H., Osama, A.K., Makky, E.A, Rahim, M.H, Ali, N.H.M., & Hazrudin, N.D. (2019). Phytochemical screening, antimicrobial and antioxidant efficacy of some plant extracts and their mixtures. IOP Conference Series: Earth and Environmental Science, 346, 012003. https://doi.org/10.1088/1755-1315/346/1/012003
Machado, C.A., Oliveira, F.O., de Andrade, M.A., Hodel, K.V.S., Lepikson, H., & Machado, B.A.S. (2022). Steam distillation for essential oil extraction: an evaluation of technological advances based on an analysis of patent documents. Sustainability, 14(12), 7119. https://doi.org/10.3390/su14127119
Martins, R., Barbosa, A., Advinha, B., Sales, H., Pontes, R., & Nunes, J. (2023). Green extraction techniques of bioactive compounds: a state-of-the-art review. Processes, 11(8), 2255. https://doi.org/10.3390/pr11082255
Moretto, J.A., de Souza, A.O., Berneira, L.M., Brigagão, L.G.G., de Pereira, C.M.P., Converti, A., & Pinto, E. (2022). Microwave-assisted extraction of fatty acids from cultured and commercial phytoplankton species. Applied Sciences, 12(5), 2407. https://doi.org/10.3390/app12052407
Naviglio, D., Trifuoggi, M., Varchetta, F., Nebbioso, V., Perrone, A., Avolio, L., De Martino, E., Montesano, D., & Gallo, M. (2023). Efficiency of recovery of the bioactive principles of plants by comparison between solid–liquid extraction in mixture and single-vegetable matrices via maceration and RSLDE. Plants, 12(16), 2900. https://doi.org/10.3390/plants12162900
Palmieri, S., Pellegrini, M., Ricci, A., Compagnone, D., & Lo Sterzo, C. (2020). Chemical composition and antioxidant activity of thyme, hemp and coriander extracts: a comparison study of maceration, soxhlet, UAE and RSLDE techniques. Foods, 9(9), 1221. https://doi.org/10.3390/foods9091221
Ranjha, M.M.A.N., Irfan, S., Lorenzo, J.M., Shafique, B., Kanwal, R., Pateiro, M., Arshad, R.N., Wang, L., Nayik, G.A., Roobab, U., & Aadil, R.M. (2021). Sonication, a potential technique for extraction of phytoconstituents: a systematic review. Processes, 9(8), 1406. https://doi.org/10.3390/pr9081406
Oubannin, S., Bijla, L., Ahmed, M.N., Ibourki, M., El Kharrassi, Y., Devkota, K., Bouyahya, A., Maggi, F., Caprioli, G., Sakar, E.H., & Gharby, S. (2024). Recent advances in the extraction of bioactive compounds from plant matrices and their use as potential antioxidants for vegetable oils enrichment. Journal of Food Composition and Analysis, 128, 105995. https://doi.org/10.1016/j.jfca.2024.105995
Usman, M., Nakagawa, M., & Cheng, S. (2023). Emerging trends in green extraction techniques for bioactive natural products. Processes, 11(12), 3444. https://doi.org/10.3390/pr11123444
Uwineza, P.A., & Waśkiewicz, A. (2020). Recent advances in supercritical fluid extraction of natural bioactive compounds from natural plant materials. Molecules, 25(17), 3847. https://doi.org/10.3390/molecules25173847
Published
Data Availability Statement
Not applicable.
Issue
Section
License
Copyright (c) 2025 Yanelis Chongo (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.